解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Sugarcane white leaf (SCWL)-diseased sugarcane plants collected from Udornthani Province, in north-eastern Thailand, were the source for tissue culture experiments. Explants from axillary buds, meristem tips, and leaves grew optimally in Murashige-Skoog medium containing 0.5 mg/l alpha-naphthaleneacetic acid, 0.5 mg/l...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0847-2
更新日期:2004-11-01 00:00:00
abstract::A simple and reliable method was developed for isolating pollen nuclei from Brassica napus and Triticum aestivum for DNA analysis using flow cytometry. The nuclei were released from pollen by ultrasonic treatment. The isolated nuclei following filtration through nylon mesh and a purification procedure were suitable fo...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0830-y
更新日期:2004-10-01 00:00:00
abstract::A protocol for Agrobacterium-mediated transformation with mannose selection was developed for cotyledon petiole, hypocotyl and leaf explants of tomato (Lycopersicon esculentum L. Mill). More than 400 transgenic plants from three tomato varieties were selected with 1% mannose in combination with 0.1-0.5% glucose. Avera...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0809-8
更新日期:2004-10-01 00:00:00
abstract::Phytohormones are essential signal compounds in the regulation of stress-related and defense-related genes. However, there is no clear evidence for any effect of these signal molecules and biotic elicitors on the regulation of the SALT gene in suspension-cultured rice cells. We characterized the expression of a SALT g...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0836-5
更新日期:2004-10-01 00:00:00
abstract::We have developed a plant- Escherichia coli pASV shuttle vector from the essential elements of the Ageratum yellow vein virus (AYVV). The geminivirus vector contains the AYVV genome with the coat-protein deletion, the E. coli vector backbone of pUC19, a unique cloning site and gene expression cassettes for plant selec...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0792-0
更新日期:2004-08-01 00:00:00
abstract::Most infections of plant roots are initiated in the region of elongation; the mechanism for this tissue-specific localization pattern is unknown. In alfalfa expressing PsUGT1 antisense mRNA under the control of the cauliflower mosaic virus (CaMV) 35S promoter, the cell cycle in roots is completed in 48 h instead of 24...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-004-0787-x
更新日期:2004-07-01 00:00:00
abstract::The purpose of this study was to develop a new micropropagation system for Tylophora indica, an important medicinal plant in India, using root explants as starting material. Root explants cultured on MS medium supplemented with 6-benzyladenine (BA) or 2-isopentyladenine (2iP) produced organogenic nodular meristemoids ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0753-z
更新日期:2004-05-01 00:00:00
abstract::A protocol was developed for biolistic transformation of hybrid bermudagrass cv. TifEagle using the bar gene. TifEagle is an ultradwarf used exclusively on golf greens. Herbicide resistance should serve as a useful management tool, especially if methyl-bromide is unavailable for fumigation prior to plant establishment...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0736-0
更新日期:2004-03-01 00:00:00
abstract::Pyrolysis mass spectrometry (PyMS) is a rapid, simple, high-resolution analytical method based on thermal degradation of complex material in a vacuum and has been widely applied to the discrimination of closely related microbial strains. Leaf samples of six species and one variety of higher plants (Rosa multiflora, R....
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0714-6
更新日期:2004-02-01 00:00:00
abstract::A DNA fragment encoding a 12-amino acid (aa) HIV-1 Tat transduction peptide fused to a 90-aa murine rotavirus NSP4 enterotoxin protein (Tat-NSP4(90)) was transferred to Solanum tuberosum by Agrobacterium tumefaciens-mediated transformation. The fusion gene was detected in the genomic DNA of transformed plant leaf tiss...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0697-3
更新日期:2004-01-01 00:00:00
abstract::This report describes the successful plant regeneration via somatic embryogenesis from immature zygotic embryos of Cryptomeria japonica D. Don. For the induction of embryogenic tissue, we determined that the optimal medium contained N6-benzyladenine and 2,4-dichlorophenoxyacetic acid. Immature zygotic embryos that wer...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0687-5
更新日期:2003-11-01 00:00:00
abstract::Recently, five novel fluorescent proteins have been isolated from non-bioluminescent species of reef-coral organisms and have been made available through ClonTech. They are AmCyan, AsRed, DsRed, ZsGreen and ZsYellow. These proteins are valuable as reporters for transformation because they do not require a substrate or...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0690-x
更新日期:2003-11-01 00:00:00
abstract::A reliable and efficient system for transformation and regeneration of 'Chardonnay' (Vitis vinifera L.) plants via microprojectile bombardment was developed. Improvements over the previous biolistic transformation system included: (1) the use of gold particles for bombardment; (2) step-wise selection at 10 then 15 mg/...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0682-x
更新日期:2003-11-01 00:00:00
abstract::Eustoma grandiflorum Shinn requires vernalization for the induction of stem elongation and flowering. To investigate the role of gibberellins (GAs) in vernalization, the expression levels of genes encoding enzymes of GA biosynthesis, copalyl diphosphate synthetase, GA 20-oxidase and GA 3 beta-hydroxylase, were examine...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0672-z
更新日期:2003-09-01 00:00:00
abstract::Taro bacilliform virus (TaBV) is a pararetrovirus of the genus Badnavirus which infects the monocotyledonous plant, taro ( Colocasia esculenta). A region of the TaBV genome spanning nucleotides 6,281 to 12 (T1200), including the 3' end of open reading frame 3 (ORF 3) and the intergenic region to the end of the tRNA(me...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0621-x
更新日期:2003-08-01 00:00:00
abstract::Randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers were used to analyse the genetic diversity of Portuguese Prunus dulcis cultivars and their relationship to important foreign cultivars. Of the primers tested, 6 (out of 60) RAPD and 5 (out of 18) ISSR primers were selected for th...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0659-9
更新日期:2003-08-01 00:00:00
abstract::The objective of this study was to correlate the time that DNA synthesis first occurs in haploid microspores of barley with cell cycle and plant morphological stages and to subsequently assess the influence of pretreatments on DNA synthesis at different stages of microspore development. Spikes with microspores in earl...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0635-4
更新日期:2003-07-01 00:00:00
abstract::Site-specific recombination has been developed into a genetic engineering tool for higher eukaryotes. The manipulation of newly introduced DNA is now possible in the course of genetic transformation procedures, thus making the process more predictable and reliable. Also, a wide variety of chromosomal rearrangements us...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s00299-003-0616-7
更新日期:2003-06-01 00:00:00
abstract::The present protocol was aimed at establishing a routine transformation procedure via Agrobacterium tumefaciens for an important Oncidium orchid cultivar. An expression vector containing hptII and gusA genes driven by the cauliflower mosaic virus (CaMV) 35S promoter was successfully introduced into the Oncidium orchid...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0614-9
更新日期:2003-06-01 00:00:00
abstract::Most perennial plants must pass through a long juvenile phase of vegetative development before they are capable of flowering. We have developed a method specifying inflorescence proliferation to bypass juvenility and maintain the adult phase. Bamboo ( Bambusa edulis) inflorescences were amplified by incubation in Mura...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0571-3
更新日期:2003-06-01 00:00:00
abstract::Self-incompatibility (SI) discriminating self- and non-self pollen is regulated by S-locus genes in Brassica. In most of the S haplotypes, a highly polymorphic S-locus glycoprotein ( SLG) gene is tightly linked to genes for the SI determinants, S-receptor kinase ( SRK) and SP11, although the precise function of SLG in...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0598-5
更新日期:2003-06-01 00:00:00
abstract::The regeneration of somatic seedlings from selected 100-year-old cork oak trees is reported. The induction of somatic embryogenesis from leaves of epicormic shoots was significantly affected by genotype, harvesting time and their interaction. Leaves from all five selected trees produced somatic embryos when the segmen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-003-0604-y
更新日期:2003-04-01 00:00:00
abstract::Cryopreservation has been shown to improve the frequency of virus elimination - specifically cucumber mosaic virus and banana streak virus - from banana ( Musa spp.) plants. To understand the mode of action of cryopreservation for the eradication of viral particles, we examined the ultrastructure of meristem tips at e...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-002-0537-x
更新日期:2003-03-01 00:00:00
abstract::Additional virulence (vir) genes in Agrobacterium tumefaciens and sonication were investigated for their impact on transformation efficiency in loblolly pine (Pinus taeda L.). Mature zygotic embryos of loblolly pine were co-cultivated with disarmed A. tumefaciens strain EHA105 containing either plasmid vector pCAMBIA1...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-002-0550-0
更新日期:2003-02-01 00:00:00
abstract::The parameters for optimal regeneration of seven commercial strawberry cultivars were tested using a range of explants and culture conditions. Efficient levels of regeneration--those needed to carry out transformation experiments--with the cultivars Calypso, Pegasus, Bolero, Tango and Emily were achieved with leaf dis...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-002-0530-4
更新日期:2003-01-01 00:00:00
abstract::The expression of green fluorescent protein (GFP) and its inheritance were studied in transgenic oat ( Avena sativa L.) plants transformed with a synthetic green fluorescent protein gene [sgfp(S65T)] driven by a rice actin promoter. In vitro shoot meristematic cultures (SMCs) induced from shoot apices of germinating m...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s00299-002-0542-0
更新日期:2003-01-01 00:00:00
abstract::Quantitative real-time polymerase chain reaction (PCR) assays were designed that enabled the zygosity of transgenes in soybean [Glycine max (L.) Merrill] and peanut (Arachis hypogaea L.) to be determined. The two zygosity assays, based on TaqMan technology that uses a fluorogenic probe which hybridizes to a PCR target...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990100326
更新日期:2001-07-01 00:00:00
abstract::The green fluorescent protein (GFP) from the jellyfish Aequorea victoria has proven to be a powerful tool in plant genetic transformation studies. This paper reviews the history and the progression of the expression of GFP variants in transgenic plants. The distinguishing features of the most useful GFPs, such as thos...
journal_title:Plant cell reports
pub_type: 杂志文章,评审
doi:10.1007/s002990100346
更新日期:2001-07-01 00:00:00
abstract:: The influences of light conditions, sucrose and ethylene on in vitro formation and storability of onion (Allium cepa L.) bulblets were studied in various accessions. Light, sucrose and ethylene influenced bulb formation. Storability was primarily enhanced by a high sucrose concentration (100 g/l) in the culture mediu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000295
更新日期:2001-02-01 00:00:00
abstract:: Somatic embryogenesis and whole plant regeneration were achieved in callus cultures derived from immature zygotic embryos of Acacia mangium. Embryogenic callus was induced on MS medium containing combinations of TDZ (1-2 mg/l), IAA (0.25-2 mg/l) and a mixture of amino acids. Globular embryos developed on embryogenic ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000288
更新日期:2001-01-01 00:00:00
abstract:: Embryogenic nucellar callus cultures of different Citrus species and cultivars growing in hormone-free medium were transferred to medium containing either sucrose or glycerol as the only carbohydrate source. Glycerol has been reported to induce further development of Citrus somatic embryos, while in the presence of s...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000280
更新日期:2001-01-01 00:00:00
abstract:: Cells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2 M glycerol and 0.4 M sucrose for 15 min at room temperature and then dehydrated with a vitrification solution (PVS2) for 1-3 h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazoliu...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000255
更新日期:2000-12-01 00:00:00
abstract:: A novel procedure has been developed to produce rice (Oryza sativa L.) tolerant to the herbicide phosphinothricin (PPT) by means of in vitro selection. First, sublethal and lethal concentrations of PPT on 7-day-old seedlings were determined and morphogenetic events in response to the PPT treatment evaluated. Differen...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000276
更新日期:2000-12-01 00:00:00
abstract:: The potential of ultrasonic standing waves for trapping suspended particles was utilized successfully for differential size fractionation of plant somatic embryos. In a flow-through resonator equipped with a 200-kHz piezoceramic transducer, embryos of different sizes corresponding to different developmental stages co...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000234
更新日期:2000-11-01 00:00:00
abstract::LITHOSPERMUM ERYTHRORHIZON: , which are capable of producing red pigments, have been established. The red pigments were formed on the stems of L. erythrorhizon shoots cultured both on solid and in liquid media without phytohormones at 25 °C in the dark. Thin-layer chromatography, high-performance liquid chromatography...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000237
更新日期:2000-11-01 00:00:00
abstract:: A system for barley transformation via polyethyleneglycol-mediated DNA uptake into protoplasts isolated directly from scutella and the regeneration of transgenic plants is reported. Scutellum protoplasts (cv. Clipper, an Australian malting cultivar) were co-transformed with plasmids Act 1-DGUS, containing the marker ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000220
更新日期:2000-10-01 00:00:00
abstract:: Synthesis of five different Sudan-β-D-glucuronides (I, II, III, IV, and RedB) was performed by condensation of a set of red Sudan diazo dyes with methyl (1-deoxy-2,3,4-tri-O-acetyl-1-trichloroacetimidoyl-α-D-glucopyran)uronate. After the acid and alcohol groups had been deprotected, the resulting compounds were used ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000219
更新日期:2000-10-01 00:00:00
abstract::Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000198
更新日期:2000-09-01 00:00:00
abstract::The interior tissues of the alfalfa (Medicago sativa) root nodule differ in form and function from the peripheral layers. The interior tissues are specialized for the fixation of nitrogen in cells infected by rhizobia. In contrast, the peripheral nodule tissues perform roles that assist the interior tissues: they prov...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002990000216
更新日期:2000-09-01 00:00:00
abstract:: This study was conducted to detect the optimum conditions for DNA transfer into mature embryos of barley via electroporation. Cultured mature embryos of barley were directly electroporated in the presence of the pBI 121 vector carrying both the β-glucuronidase (GUS) and neomycin phosphotransferase II (npt II) genes. ...
journal_title:Plant cell reports
pub_type: 杂志文章
doi:10.1007/s002999900182
更新日期:2000-07-01 00:00:00